By insuring the test is fair, we will gain accurate results. From basic knowledge, the rate of reaction will affect by the concentration of the reactant. Set in test tube rack where the tube is clearly visible. Read the information carefully and think of a related research question that you would like to investigate. This type of reaction where a molecule is broken down into smaller pieces is called an anabolic reaction. The volume of oxygen can be measured by taking the initial and final reading of water in the pipette. This is important because Catalase are abundant in humans and all organisms and it is a basic example of what an enzyme's function is.
They are used to speed up specific reactions in the cells. Yes, catalase functions best at the same low pH and temperature as occurs in the mammalian tissue. Observation of Catalase Activity 1. Cover the crushed potato when not being used. The enzyme and substrate are held together in an enzyme- substrate complex by hydrophobic bonds, hydrogen bonds,.
Some enzymes… 1946 Words 8 Pages The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Now that I have carried out the repeats and plotted the points on the rate of reaction graph, I can see that the graph is in fact clearly linear. From the curves of best fit, I can also see that there were no anomalous results, only some results which were slightly above or below the curve, though they were not excessively distorted. It could be repeated more times to help get rid of any anomalies. This experiment could be improved in a number of ways. These parameters are water, Hydrogen Peroxide, Catalyse and the duration of the reactions. This is because the maximum number of reactions are being done at once so any extra substrate molecules have to wait until some of the active sites become available.
Disclaimer This is a real A-level school project and as such is intended for educational or research purposes only. Drop a piece of uncooked hamburger into test tube 4 contains H2O2. Enzymes such as Catalase are protein molecules that are found in living cells. It will also rule out the fact that if I have to do my procedures in different rooms and on different days, the temperature in the room might change. Supporting knowledge: Enzymes function as biological catalysts, increasing the rate of reactions that take place within organisms. Hydrogen peroxide is a molecule that can attack sulphur atoms and metal ions in protein molecules, and with the aid of iron ions is potentionally mutagenic. Reasearch and rationale Hypothesis: Catalase will break down hydrogen peroxide in water and hydrogen.
The results when plotted resulted in a bell shaped curve. By measuring the distance of the water move in. This meant that there were less successful collisions overall see Fig. Also, they can speed up Metabolic reactions without being changed or used up. Because of this, it is very important to ensure a constant temperature is maintained. Dependent Variable The dependent variable are the values, which depend of the independent variable.
Background knowledge: Enzymes are organic catalysts composed of proteins that assist organisms in facilitating metabolic reactions without undergoing any change themselves. Pour 80 ml of H 2O 2 into a small beaker or test tube. Common catalysts include manganese dioxide, silver, and platinum. There are procedures in place to prevent these problems, wearing eye protection and washing hands after the use of hydrogen peroxide. I carried out the above experiment and these results were obtained. They are used to speed up specific reactions in the cells.
Variables Temperature: Temperature is an extremely important variable to consider, as it can affect the rate of reaction in a number of ways. This breakdown happens rapidly in the present of the Catalase and Oxygen gas evolves rapidly. Hydrogen peroxide is a clear liquid, slightly more viscous than water. In supplement, some mechanical facets of the toxicit. Enzymes such as amylase, an enzyme that breaks down carbohydrates, work by means of surface catalysis. It would bubble quickly no matter what.
Different enzymes have different optimum temperatures, depending on the organism and environment they have evolved in. It is called an anabolic reaction. I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order to ensure a fair test. For a reaction to happen the reactant particles must collide. You will now dilute the peroxide in order to change its concentration. Introduction This is an A-level biology project. In the second set of 4 test tubes.
This enabled me to find out if each concentration, based on the number of molecules of substrate in each decrease of 10%, was similar or showed a pattern which I failed to identify with my previous results. Hydrogen Peroxide broken down will produce Water and Oxygen. Supporting knowledge: Enzymes function as biological catalysts, increasing the rate of reactions that take place within organisms. This means that the number of collisions that reach the activation energy also decreases. Further Experiments Consequently, I have decided to do further experiments with concentrations of 10% and 30% hydrogen peroxide.
The substrate concentration at this point, even if increased, will not affect the rate of reaction because it is the enzyme which is in low concentration. In this particular experiment we will be using diluted Hydrogen Peroxide as we will be using a lot. Most intracellular enzymes work best in neutral conditions, i. Hydrogen peroxide is an irritant, and a powerful oxidising agent and bleach. There were many hazards with this experiment. Enzymes, which in the experiment was peroxidase extracted from turnip, are protein catalysts that break up the substrate, which in this experiment was hydrogen peroxide. I carried out a suitable control experiment to prove that it was the enzyme in fact breaking down the hydrogen peroxide.